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AAT Bioquest

FAQ

Posted on April 19, 2024
What factors should I consider when choosing a DNA extraction method?
Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesDNA and RNA QuantitationDNA extractionNucleic AcidsNucleuscell lysis


Posted on April 19, 2024
What is the dialysis method in protein isolation?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is the purpose of dialysis in protein isolation?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What factors affect the rate of dialysis?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the principles of dialysis?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is a typical dialysis procedure
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the methods used for protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
How do I optimize protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the differences between desalting and buffer exchange?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the applications of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is the process of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the benefits of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on April 12, 2024
How do I isolate nuclei before my ChIP experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleuscell lysis


Posted on April 10, 2024
What are the components of an extraction buffer?
Chemical ReagentsBuffer Preparations and RecipesDNA extractionExtractioncell lysisdetergent


Posted on April 10, 2024
What is the significance of an extraction buffer?
Chemical ReagentsBuffer Preparations and RecipesDNA extractionExtractioncell lysisdetergent


Posted on December 22, 2023
How do I lyse cells using physical methods?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on December 22, 2023
Why is lysis important in DNA extraction?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on December 22, 2023
How do I lyse cells using chemical methods?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on September 7, 2023
How is proteinase K involved in cell lysis?
Antibodies and ProteomicsDNA and RNAEnzymesNucleic AcidsProteincell lysis


Posted on September 30, 2020
How do I lyse cells?
Cell Cycle AnalysisCellular Structures and OrganellesDNA and RNAbuffercell lysis


Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent


Posted on October 22, 2019
How to lyse adherent cells?
cell lysisadherent cells


Posted on October 21, 2019
How to lyse cells?
cell lysis


Posted on September 6, 2019
What does an assay buffer do?
cell culturecell lysisisotonicbuffer


Posted on August 26, 2019
Why are cells lysed?
Cell AssayCell ViabilityWestern Blotcell lysishomogenouscentrifugeIHCSignal Amplificationantibody labelingimmunohistochemistry


Posted on August 21, 2019
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex


Posted on February 26, 2018
How can I lyse my cells without lysing the nuclear membrane?
cell lysisCell Assay